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What is Nucleic Acid Amplification mean?
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete copies or partial copies) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to many of the procedures used in genetic testing and research, including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and criminal forensics.
The majority of PCR methods rely on thermal cycling. Thermal cycling exposes reactants to repeated cycles of heating and cooling to permit different temperature-dependent reactions – specifically, DNA melting and enzyme-driven DNA replication. PCR employs two main reagents – primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called nucleic acid denaturation. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA. The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides, the building blocks of DNA. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified.
Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. If the polymerase used was heat-susceptible, it would denature under the high temperatures of the denaturation step. Before the use of Taq polymerase, DNA polymerase had to be manually added every cycle, which was a tedious and costly process.
Applications of the technique include DNA cloning for sequencing, gene cloning and manipulation, gene mutagenesis; construction of DNA-based phylogenies, or functional analysis of genes; diagnosis and monitoring of genetic disorders; amplification of ancient DNA; analysis of genetic fingerprints for DNA profiling (for example, in forensic science and parentage testing); and detection of pathogens in nucleic acid tests for the diagnosis of infectious diseases.
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National Anthropological Archives | Academic & Science |
N Acetylaspartic acid | Academic & Science |
Nucleic Acid Amplification | Academic & Science |
National Academy of Arbitrators | Academic & Science |
National Alumni Association | Academic & Science |
Northwestern Alumni Association | Academic & Science |
National Association of Accountants (now Ima) | Accounts and Finance |
Narrabri | Airport Code |
National Association of Accountants | Business |
National Apartment Association | Business |
National Aquaculture Association | Business |
Naphtalic Acetic Acid | Chemistry |
National AfterSchool Association | Community |
National Association of Appraisers | Community |
Newburyport Art Association | Community |
National Aeronautics Association | Community |
National Aphasia Association | Community |
Newspaper Association of America | Community |
National Aerosol Association | Community |
National Aeronautic Association | Community |
National Auctioneers Association | Community |
National Algae Association | Community |
Nebraska Arborists Association | Community |
Next Available Agent | Computing |
Number of Attributes Accesses | Computing |
Number of All Associations | Computing |
Network Audio Adapter | General |
National Apartment | General |
North American Arms | General |
Not An Answer | General |
National Aviation Authority | General |
Nevada Alternate Assessment | General |
Not Another Acronym | General |
Network Attached Audio | General |
Negative Alien Agenda | General |
North American Aviation | General |
N Acetyl Aspartate | General |
Northwest Automotive Alliance | General |
Nippon Denso Abs Actuator | General |
National Automobile Association | General |
Norwegian Automobile Association | General |
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National Auto Academy | General |
Newera Automotive Academy | General |
Nimrokko Animal Army | General |
Never Abbreviate Again! | General |
Nutrition Academic Award | General |
National Aviation Academy | General |
not an actuary | General |
North America Administrators | General |
Network Address Authority | Governmental |
Not Army Aviation | Governmental |
Neighborhood Assistance Act | Governmental |
Newly Accessible Area | Governmental |
National Airspace Analysis | Governmental |
North American Alliance | Governmental |
No Acronym Assigned | Governmental |
Network Addressing Authority | Governmental |
National Agents Alliance | Governmental |
New America Alliance | Governmental |
Name Address Authority | Governmental |
No Apology Accepted | Internet |
Nothing At All | Maths |
No apparent abnormalities | Medical |
National Autism Association | Medical |
Neutron Activation Analysis | Physics Related |
Narrabri, New South Wales, Australia | Regional |
National Archery Association | Sports |
Newton Athletic Association | Sports |
Posted on 18 Sep 2024, this text provides information on Miscellaneous in Academic & Science related to Academic & Science. Please note that while accuracy is prioritized, the data presented might not be entirely correct or up-to-date. This information is offered for general knowledge and informational purposes only, and should not be considered as a substitute for professional advice.
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